Journal: Nature Communications
Article Title: A germline-to-soma signal triggers an age-related decline of mitochondrial stress response
doi: 10.1038/s41467-024-53064-0
Figure Lengend Snippet: a , b qRT-PCR analysis of Gli1, Hhip, and Smo mRNA levels in seven-month-old C57BL/6J male ( a ) and female ( b ) mice treated with DMSO or itraconazole. c , d qRT-PCR analysis of Chop and Asns mRNA levels in seven-month-old C57BL/6J male ( c ) and female ( d ) mice treated with DMSO or oligomycin. e , f Weight loss in seven-month-old C57BL/6J male ( e ) and female ( f ) mice during oligomycin treatment. g Litter size of seven-month-old C57BL/6J females treated with DMSO or itraconazole, mated with males of the same treatment group. h , i Serum AMH ( h ) or testosterone ( i ) levels assessed by ELISA in mice treated with DMSO or itraconazole. j – l Pearson’s correlation of GLI1, GLI2, and UPR mt mRNA levels in monkey ( j ), rat ( k ) and human ( l ) tissues. Blue indicates a positive correlation and white indicates a negative correlation. The intensity of the colors corresponds to the correlation coefficient. m Pearson’s correlation of GLI1, GLI2, GLI3, and human milk mRNA levels in human breast tissues. Blue indicates a positive correlation and white indicates a negative correlation. The intensity of the colors corresponds to the correlation coefficient. Error bars indicate mean ± SD. n represents the number of mice. p values were assessed using a two-tailed t test. Source data are provided as a Source Data file.
Article Snippet: Tissue testosterone levels were measured using an ELISA kit (CUSABIO, CSB-E05101m).
Techniques: Quantitative RT-PCR, Enzyme-linked Immunosorbent Assay, Two Tailed Test